《植物生理学报》 2009, 45(11): 1075-1080

辣椒果实中CaRIN 转录因子的克隆及其表达分析

胡宗利, 王燕, 王翊, 陈国平*

重庆大学生物工程学院, 重庆400044

通信作者：陈国平；E-mail: chenguoping@cqu.edu.cn；Tel: 023-65112674

摘　要：

根据BLAST获得的表达序列标签设计引物, 从辣椒中分离并鉴定了一个新型转录因子, 命名为CaRIN。与已知MADS- box 蛋白对比表明, CaRIN 分别与番茄 LeMADS-RIN 和矮牵牛 FBP4 有 85% 和 77% 的同源性, 并且具有典型的 MADS-box 类转录因子的特征区域。采用半定量RT-PCR进行特异性表达分析, 结果表明CaRIN在破色期和红熟期辣椒果实中大量表 达, 而在绿熟期辣椒果实和其他辣椒器官中几乎不表达; 同时进行了DNA结合位点和系统发生等生物信息学分析, 这些结 果都表明CaRIN 可能参与辣椒果实成熟的调控。

关键词：辣椒; CaRIN; MADS-box; 克隆; 半定量 RT-PCR

收稿：2009-08-11   修定：2009-10-20

资助：国家自然科学基金(30771463, 30871709)和重庆大学研究生创新团队建设项目(200909B1007)。

Cloning and Analysis of Expression of CaRIN Transcription Factor from Pepper (Capsicum annuum L. cv. Bukang) Fruit

HU Zong-Li, WANG Yan, WANG Yi, CHEN Guo-Ping*

College of Bioengineering, Chongqing University, Chongqing 400044, China

Corresponding author: CHEN Guo-Ping; E-mail: chenguoping@cqu.edu.cn; Tel: 023-65112674

Abstract:

Specific primers were designed based on the EST sequences which were obtained according to BLAST. A novel transcription factor CaRIN was isolated and characterized from the chili pepper (Capsicum annuum L. cv. Bukang). Compared with known MADS-box proteins, CaRIN had 85% and 77% sequence omology with LeMADS-RIN from tomato and FBP4 from petunia, respectively, and contained typical structural characteristics of MADS-box transcription factors. Analysis of tissue-specific expression of CaRIN was performed by semi-quantitative RT-PCR. The result showed that CaRIN mRNA was existing mainly in breaker and red ripening fruits, but hardly detectable in mature green fruit and other organs of pepper. Bioinformatic analysises, such as DNA-binding residues and phylogenetic analysis, suggested that CaRIN were involved in regulating pepper fruit ripening.

Key words: pepper; CaRIN; MADS-box; cloning; semi-quantitative RT-PCR